The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. Vii. the Methylation of Ribonucleic Acid.
نویسندگان
چکیده
Preceding publications from this laboratory have shown that the methylation of soluble ribonucleic acid is catalyzed by at least six discrete enzymes (l-3) which have been purified from extracts of Escherichia coli W. Two of these enzyme preparations catalyze the formation of l-methylguanine residues in SRNA,1 while a third produces 7-methylguanine. The others catalyze the methylation of uracil, cytosine, and adenine residues of S-RNA, yielding thymine, 5-methylcytosine, 2-methyladenine, 6-methylaminopurine, and 6-dimethylaminopurine, respectively. The work presented here describes the properties of these enzymes which catalyze the methylation of different bases of S-RNA. It has been found that each enzyme acts independently, and in all cases, when the reaction comes to a stop with a particular enzyme, further methylation is resumed only after addition of more acceptor S-RNA. The amount of methylated bases present in S-RNA is relatively small. The reactions described here may account for this finding, since there appear to be a limited number of methylation sites for a given enzyme and S-RNA.
منابع مشابه
The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid V. PURIFICATION AND PROPERTIES OF THE DEOXYRIBONUCLEIC ACID-METHYLATING ACTIVITY OF ESCHERICHIA COLP
The classical investigations of Wyatt (2) and Dunn and Smith (3) established the existence of 5-methylcytosine and 6-methylaminopurine as constituents of the deoxyribonucleic acid of higher plants and animals, and of bacteria and bacterial viruses, respectively. Until recently, the biochemical pathways for the incorporation of these “trace bases” into deoxyribonucleic acid was unknown although ...
متن کاملThe Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. v. Purification and Properties of the Deoxyribonucleic Acid-methylating Activity of Escherichia Coli.
The classical investigations of Wyatt (2) and Dunn and Smith (3) established the existence of 5-methylcytosine and 6-methylaminopurine as constituents of the deoxyribonucleic acid of higher plants and animals, and of bacteria and bacterial viruses, respectively. Until recently, the biochemical pathways for the incorporation of these “trace bases” into deoxyribonucleic acid was unknown although ...
متن کاملMethylation of ribonucleic acid and deoxyribonucleic acid and tumour induction in livers of hypophysectomized rats treated with dimethylnitrosamine.
متن کامل
The Isolation and Characterization of Adenosine Monophosphate-rich Polynucleotides Synthesized by Soybean Hypocotyl Cells: Their Relation to Messenger Ribonucleic Acid.
Plant ribonucleic acids which have high adenosine monophosphate concentrations were studied. Purified deoxyribonucleic acid-like ribonucleic acid and tenaciously bound ribonucleic acid fractions both contained poly-adenosine monophosphate sequences (those from the latter being longer than those from the former); without these poly-adenosine monophosphate sequences their base compositions were t...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 241 9 شماره
صفحات -
تاریخ انتشار 1964